Effect of Adding Probiotic Yeast and Fermentation Time on Probiotic Tempeh Characteristics

Authors

  • Mari'ah Qibty Agricultural Product Technology, Faculty of Agroindustry, Mercu Buana Yogyakarta University
  • WisnuYulianto Agricultural Product Technology, Faculty of Agroindustry, Mercu Buana Yogyakarta University
  • Pujimulyani Agricultural Product Technology, Faculty of Agroindustry, Mercu Buana Yogyakarta University

DOI:

https://doi.org/10.26486/jfat.v1i1.3390

Keywords:

Probiotic yeast, Saccharomyces boulardi, tempeh

Abstract

Tempeh is one of the foods made from soybean seeds or other ingredients that are processed through fermentation. Tempeh currently circulating in the community does not contain probiotic yeast, the addition of probiotic yeast is intended to provide probiotic properties to tempeh. From previous studies, yeast isolates from radishes and tomatoes have been identified as potential probiotic yeasts. This study aimed to determine the effect of adding the type of probiotic yeast isolate and fermentation time on the number of fungi, the number of yeasts, pH, antioxidant activity, and the level of preference by panelists for the resulting probiotic tempeh. This study used a completely randomized design with a factorial pattern. The first factor was the type of probiotic inoculum (Saccharomyces boulardii, yeast isolate LBK K, yeast isolate TMT 2), and the second factor was incubation time (0 hours, 24 hours, and 48 hours). The analysis included the number of fungus and yeast (plate count agar), antioxidant activity (radical scavenging activity (%), and preference level for probiotic tempeh. The data obtained were analyzed for variance (ANOVA) at a 95% confidence level. If there was a significant difference between each treatment was continued with Duncan's multiple range test (DMRT). The results showed that adding probiotic yeast type and a long incubation time affected the microbiological properties, antioxidant activity, and panelist preference. The most preferred tempeh was tempeh with the addition of LBK K isolate with an incubation time of 48 hours with a pH of 5.46, the total number of fungi was 6.62 log CFU/g, the total amount of yeast was 8,63 log CFU/g, the antioxidant activity was 18.58% RSA. Tempeh, with the addition of LBK K yeast isolate, has the potential as a probiotic food.

References

Barus, T., Lydia, Natalia W. 2010. Dominant Microbiota and Its Role in the Taste of Cassava Tape. Biota Vol. 16 (2): 354-361, June 2011. ISSN 0853-8670. Faculty of Technology. The Atma Jaya Catalytic University of Indonesia. Jakarta.

Buckle, KA., Edwards, Fleet, and Wootton. 1987. Food Science. UI-Press, Jakarta.

Espinosa and Ruperez. 2006. Soybean oligosaccharides Potential as new ingredients in functional food. Nutr. Hosp. v.21 n.1 Madrid ene.-feb. 2006.

Fahrizal, AG 2022. Characteristics and Level of Likeness of Glutinous Tapai Ice Cream and Probiotic Cassava. Jurnal Teknologi dan Industri Pangan Unisri. Vol 7:2 (153-165)

Fardiaz, S. 1989. Microbiological Analysis of Food. Department of Education and Culture. Bogor: PAU Food and Nutrition.

Gibson, GR and MB Roberfroid. 2008. Prebiotics: Concept, Definition, Criteria, Methodologies, and Products. Handbook of Prebiotics. CRC Press. New York

Hartiningrum, SY 2010. The Effect of Giving Preda and Tempe Formulas on the Old Age of Acute Diarrhea in Children Aged 6 – 24 Months Study at RSU RA. Jepara Regency Kartini Year 2010. Thesis. Semarang: Diponegoro University.

Hayati, EN 2010. Study of antioxidant activity and dietary fiber content in soybean/rice-based tempeh with the addition of Angkak and variations in fermentation time. Thesis. Faculty of Agriculture. Surakarta: UNS.

Kiers, JL, Schreuder, J., Nout, MJR and Rombousts, F. 1997. Tempe fermentation, using different substrates and pure culture states, and the effect of substrate modification on in vitro digestibility.

Kustyawati, ME 2009. Study of the Role of Yeast in Making Tempe. Agritech Journal. THP Department, Faculty of Agriculture, University of Lampung. 29:2

Marteau, PR, de Vrese, M., Cellier, CJ, and Schrezeenmeier, J., 2001. Protection from gastrointestinal diseases with the use of probiotics. Am. J. ClinNutr: 73: 430S436S.

Nurrahman, Suparmo, et al. 2012. Fungus Growth, Organoleptic Properties, and Antioxidant Activity of Black Soybean Tempe Produced with Various Types of Inoculums. Journal of Agritech 32:1.

Nuraida, L., et al. 2018. Selection of Lactic Acid Bacteria Isolates from Tempe and Tape as Probiotic Candidates. Journal of Food Technology and Industry. Bogor Agricultural Institute. 29(2):175-184.

Pangastuti, HP and Triwibowo, S. 1996. The process of making soybean tempeh. Mirror of the World of Medicine 190: 54-56.

Salminen, S., Atte, W. and Arthur. 2004. Lactic Acid Bacteria: Microbial and Functional Aspects, 3rd Edition Revised and Expanded. New York: Marcel Dekker, Inc.

Setiawati H, 2015, The Effect of Giving Tempe Porridge Diet on the Frequency of Defecation in Children with Diarrhea in the Mina Room of PKU Muhammadiyah Surakarta Hospital.

Stefani, A. 2019. Probiotic Saccharomyces boulardii As A Prevention Of Clostridium Difficile Infection Due To The Use Of Antibiotics. Anatomical Medical Journal. E-ISSN: 2614-5219. Medical School. Lampung University.

Vilijoen, BC and Greyling, T. 1995. Yeast is associated with cheddar and gouda making. International Journal of Food Microbiology 28: 79-88

Weny, A., Darmawati, Sri W. 2015. Old Fermentation Study of the Alcohol Content of Black Glutinous Tape (Oryza Sativa Glutinosa) as the Development of Student Worksheets on Conventional Biotechnology Concepts. Class XII SMA. Riau. Riau University.

Winarno, FG 2004. Kimia Pangan dan Gizi. Main Library Gramedia. Jakarta.

Yulianto, W. and Dwiyati P. 2022. Isolation and Characterization of Yeast Probiotic Potency from Various Sources. Umbrella Research Report. Yogyakarta: Mercu Buana University, Yogyakarta.

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Published

2023-11-15

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